Review



9 mer primers  (TaKaRa)


Bioz Verified Symbol TaKaRa is a verified supplier
Bioz Manufacturer Symbol TaKaRa manufactures this product  
  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 96

    Structured Review

    TaKaRa 9 mer primers
    9 Mer Primers, supplied by TaKaRa, used in various techniques. Bioz Stars score: 96/100, based on 6510 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/random+9+mer+primers/Random+Primer/pm41621163-72-13-16
    Average 96 stars, based on 6510 article reviews
    9 mer primers - by Bioz Stars, 2026-09
    96/100 stars

    Images

    Related Articles

    Synthesized:

    Article Title: Bone Marrow Mesenchymal Stem Cell‐Derived Exosomes Promote M2 Polarization and Protect Against Acute Lung Injury
    Article Snippet: .. For mRNA quantification, cDNA was synthesized using Oligo(dT) and Random 9‐mer primers (Takara, Dalian, China), with β‐actin as the internal reference. ..

    Article Title: Interleukin-6 regulates anti-arthritic effect of methotrexate via reduction of SLC19A1 expression in a mouse arthritis model
    Article Snippet: .. Synovial cells (5 × 10 4 cells/2 mL/well) were cultured in α-MEM supplemented with 10% FBS for 24 h. After this pre-culture, cells were cultured with mouse IL-6 (Peprotech, Rocky Hill, NJ, USA) and soluble mouse IL-6R (R&D Systems, Minneapolis, MN, USA), TNF-α, or MTX for 24 h. Total RNA was extracted using an RNeasy kit (Qiagen) according to the kit manufacturer's protocol. cDNA was synthesized with an Omniscript RT kit (Qiagen) using random 9-mer primers (Takara) according to the kit manufacturer's protocol. .. Quantitative real-time PCR was performed by running a TaqMan gene expression assay, targeting mouse SLC19A1, multidrug resistance protein-1 (MRP-1), breast cancer resistance protein (BCRP) , and GAPDH , on an ABI PRISM 7500 system (Applied Biosystems) according to the manufacturer's protocol.

    Article Title: Bone Marrow Mesenchymal Stem Cell‐Derived Exosomes Promote <scp>M2</scp> Polarization and Protect Against Acute Lung Injury
    Article Snippet: .. For mRNA quantification, cDNA was synthesized using Oligo(dT) and Random 9- mer primers (Takara, Dalian, China), with β- actin as the internal reference. ..

    Article Title: Anti-inflammatory Effects of Empagliflozin and Gemigliptin on LPS-Stimulated Macrophage via the IKK/NF- κ B, MKK7/JNK, and JAK2/STAT1 Signalling Pathways
    Article Snippet: The total RNAs were extracted using the RNAiso Plus reagent (Takara Bio, Otsu, Japan) according to the manufacturer's protocol. .. Complementary DNA (cDNA) was synthesized from RNA using avian myeloblastosis virus (AMV) reverse transcriptase (BEAMS Biotechnology, Seongnam, South Korea), and random 9-mer primers, then amplified qPCR using primer sets (Takara Bio Inc., Shiga, Japan) specific for mouse COX-2: CAG CAA ATC CTT GCT GTT CC (forward, F) and TGG GCA AAG AAT GCA AAC ATC (reverse, R); mouse iNOS: AGA CCT CAA CAG AGC CCT CA (F) and GGC TGG ACT TTT CAC TCT GC (R); mouse tumor necrosis factor- α (TNF- α ): TCG TAG CAA ACC ACC AAG TG (F) and AGA TAG CAA ATC GGC TGA CG (R); mouse interleukin (IL)-1 β : TCT CGC AGC AGC ACA TCA ACA (F) and CCT GGA AGG TCC ACG GGA AA (R); mouse IL-6: GAC CTG TCT ATA CCA CTT CAC (F) and GTG CAT CAT CGT TGT TCA TAC (R); mouse C-C motif chemokine ligands (CCL) 3: ACT GCC CTT GCT GTT CTT CT (F) and GTC TCT TTG GAG TCA GCG CA (R); mouse CCL4: CTC TCT CCT CTT GCT CGT GG (F) and CTC ACT GGG GTT AGC ACA GA (R); mouse CCL5: CCA TCA TCC TCA CTG CAG CC (F) and CTC TGG GTT GGC ACA CAC TT (R); and mouse C-X-C motif chemokine ligand (CXCL) 10: CCA AGT GCT GCC GTC ATT TT (F) and TCA TCA TTC TTT TTC ATC GTG GCA (R). .. Quantitative real-time PCR was performed using SYBR Green Master Mix (Takara Bio Inc., Tokyo, Japan) on a Takara TP-815 instrument.

    Article Title: Interleukin-6 regulates anti-arthritic effect of methotrexate via reduction of SLC19A1 expression in a mouse arthritis model
    Article Snippet: Total RNA was extracted using an RNeasy kit (Qiagen) according to the kit manufacturer's protocol. .. Amounts of total RNA obtained from a single whole hind limb were 4.5 to 18 μg (intact mice), 15to 60 μg (non-treated immunized mice), 9 to 60 μg (MTX-treated immunized mice), 12 to 30 μg (MR16-1-treated immunized mice), and 4.2 and 30 μg (MTX- and MR16-1-treated immunized mice). cDNA was synthesized with an Omniscript RT kit (Qiagen) using random 9-mer primers (TaKaRa, Shiga, Japan) according to the kit manufacturer's protocol. .. Quantitative real-time polymerase chain reaction (PCR) was performed by running a TaqMan gene expression assay (Applied Biosystems, Foster City, CA, USA), targeting mouse SLC19A1, IL-6, TNF-α , and glyceraldehyde-3-phosphate dehydrogenase (GAPDH) , on an ABI PRISM 7500 system (Applied Biosystems) according to the manufacturer's protocol.

    Cell Culture:

    Article Title: Interleukin-6 regulates anti-arthritic effect of methotrexate via reduction of SLC19A1 expression in a mouse arthritis model
    Article Snippet: .. Synovial cells (5 × 10 4 cells/2 mL/well) were cultured in α-MEM supplemented with 10% FBS for 24 h. After this pre-culture, cells were cultured with mouse IL-6 (Peprotech, Rocky Hill, NJ, USA) and soluble mouse IL-6R (R&D Systems, Minneapolis, MN, USA), TNF-α, or MTX for 24 h. Total RNA was extracted using an RNeasy kit (Qiagen) according to the kit manufacturer's protocol. cDNA was synthesized with an Omniscript RT kit (Qiagen) using random 9-mer primers (Takara) according to the kit manufacturer's protocol. .. Quantitative real-time PCR was performed by running a TaqMan gene expression assay, targeting mouse SLC19A1, multidrug resistance protein-1 (MRP-1), breast cancer resistance protein (BCRP) , and GAPDH , on an ABI PRISM 7500 system (Applied Biosystems) according to the manufacturer's protocol.

    Reverse Transcription:

    Article Title: The Expression of CLDN6 in Hepatocellular Carcinoma Tissue and the Effects of CLDN6 on Biological Phenotypes of Hepatocellular Carcinoma Cells
    Article Snippet: Total RNA was extracted using TRIzol reagent (Invitrogen, USA). .. 0.5 μg RNA was reverse-transcribed with PCR diagnosis kits (TianGen, China) and random 9-mer primers (TAKARA, Japan). .. PCR reaction system contains 50 ng cDNA, 1 μl of each primer and 1×PCR master Mix (TAKARA).

    Article Title: LncRNA NEAT1 Promotes the Progression of Gastric Cancer Through Modifying the miR-1224-5p/RSF1 Signaling Axis
    Article Snippet: Quantification of relative gene expression was conducted through qRT-PCR with SYBR Premix ExTaq kit (Takara, Shiga, Japan). .. One microgram of RNA was reverse-transcribed using an M-MLV Kit (Takara, Shiga, Japan) and random 9-mer primers (Takara, Shiga, Japan). .. Semiquantitative PCR was conducted using 50 ng of reverse-transcribed cDNA and 0.4 μM of each primer in a final reaction volume of 20 μL containing 1× PCR master mix (Takara, Shiga, Japan).

    Article Title: Anti-inflammatory Effects of Empagliflozin and Gemigliptin on LPS-Stimulated Macrophage via the IKK/NF- κ B, MKK7/JNK, and JAK2/STAT1 Signalling Pathways
    Article Snippet: The total RNAs were extracted using the RNAiso Plus reagent (Takara Bio, Otsu, Japan) according to the manufacturer's protocol. .. Complementary DNA (cDNA) was synthesized from RNA using avian myeloblastosis virus (AMV) reverse transcriptase (BEAMS Biotechnology, Seongnam, South Korea), and random 9-mer primers, then amplified qPCR using primer sets (Takara Bio Inc., Shiga, Japan) specific for mouse COX-2: CAG CAA ATC CTT GCT GTT CC (forward, F) and TGG GCA AAG AAT GCA AAC ATC (reverse, R); mouse iNOS: AGA CCT CAA CAG AGC CCT CA (F) and GGC TGG ACT TTT CAC TCT GC (R); mouse tumor necrosis factor- α (TNF- α ): TCG TAG CAA ACC ACC AAG TG (F) and AGA TAG CAA ATC GGC TGA CG (R); mouse interleukin (IL)-1 β : TCT CGC AGC AGC ACA TCA ACA (F) and CCT GGA AGG TCC ACG GGA AA (R); mouse IL-6: GAC CTG TCT ATA CCA CTT CAC (F) and GTG CAT CAT CGT TGT TCA TAC (R); mouse C-C motif chemokine ligands (CCL) 3: ACT GCC CTT GCT GTT CTT CT (F) and GTC TCT TTG GAG TCA GCG CA (R); mouse CCL4: CTC TCT CCT CTT GCT CGT GG (F) and CTC ACT GGG GTT AGC ACA GA (R); mouse CCL5: CCA TCA TCC TCA CTG CAG CC (F) and CTC TGG GTT GGC ACA CAC TT (R); and mouse C-X-C motif chemokine ligand (CXCL) 10: CCA AGT GCT GCC GTC ATT TT (F) and TCA TCA TTC TTT TTC ATC GTG GCA (R). .. Quantitative real-time PCR was performed using SYBR Green Master Mix (Takara Bio Inc., Tokyo, Japan) on a Takara TP-815 instrument.

    Polymerase Chain Reaction:

    Article Title: The Expression of CLDN6 in Hepatocellular Carcinoma Tissue and the Effects of CLDN6 on Biological Phenotypes of Hepatocellular Carcinoma Cells
    Article Snippet: Total RNA was extracted using TRIzol reagent (Invitrogen, USA). .. 0.5 μg RNA was reverse-transcribed with PCR diagnosis kits (TianGen, China) and random 9-mer primers (TAKARA, Japan). .. PCR reaction system contains 50 ng cDNA, 1 μl of each primer and 1×PCR master Mix (TAKARA).

    Biomarker Discovery:

    Article Title: The Expression of CLDN6 in Hepatocellular Carcinoma Tissue and the Effects of CLDN6 on Biological Phenotypes of Hepatocellular Carcinoma Cells
    Article Snippet: Total RNA was extracted using TRIzol reagent (Invitrogen, USA). .. 0.5 μg RNA was reverse-transcribed with PCR diagnosis kits (TianGen, China) and random 9-mer primers (TAKARA, Japan). .. PCR reaction system contains 50 ng cDNA, 1 μl of each primer and 1×PCR master Mix (TAKARA).

    Virus:

    Article Title: Anti-inflammatory Effects of Empagliflozin and Gemigliptin on LPS-Stimulated Macrophage via the IKK/NF- κ B, MKK7/JNK, and JAK2/STAT1 Signalling Pathways
    Article Snippet: The total RNAs were extracted using the RNAiso Plus reagent (Takara Bio, Otsu, Japan) according to the manufacturer's protocol. .. Complementary DNA (cDNA) was synthesized from RNA using avian myeloblastosis virus (AMV) reverse transcriptase (BEAMS Biotechnology, Seongnam, South Korea), and random 9-mer primers, then amplified qPCR using primer sets (Takara Bio Inc., Shiga, Japan) specific for mouse COX-2: CAG CAA ATC CTT GCT GTT CC (forward, F) and TGG GCA AAG AAT GCA AAC ATC (reverse, R); mouse iNOS: AGA CCT CAA CAG AGC CCT CA (F) and GGC TGG ACT TTT CAC TCT GC (R); mouse tumor necrosis factor- α (TNF- α ): TCG TAG CAA ACC ACC AAG TG (F) and AGA TAG CAA ATC GGC TGA CG (R); mouse interleukin (IL)-1 β : TCT CGC AGC AGC ACA TCA ACA (F) and CCT GGA AGG TCC ACG GGA AA (R); mouse IL-6: GAC CTG TCT ATA CCA CTT CAC (F) and GTG CAT CAT CGT TGT TCA TAC (R); mouse C-C motif chemokine ligands (CCL) 3: ACT GCC CTT GCT GTT CTT CT (F) and GTC TCT TTG GAG TCA GCG CA (R); mouse CCL4: CTC TCT CCT CTT GCT CGT GG (F) and CTC ACT GGG GTT AGC ACA GA (R); mouse CCL5: CCA TCA TCC TCA CTG CAG CC (F) and CTC TGG GTT GGC ACA CAC TT (R); and mouse C-X-C motif chemokine ligand (CXCL) 10: CCA AGT GCT GCC GTC ATT TT (F) and TCA TCA TTC TTT TTC ATC GTG GCA (R). .. Quantitative real-time PCR was performed using SYBR Green Master Mix (Takara Bio Inc., Tokyo, Japan) on a Takara TP-815 instrument.

    Amplification:

    Article Title: Anti-inflammatory Effects of Empagliflozin and Gemigliptin on LPS-Stimulated Macrophage via the IKK/NF- κ B, MKK7/JNK, and JAK2/STAT1 Signalling Pathways
    Article Snippet: The total RNAs were extracted using the RNAiso Plus reagent (Takara Bio, Otsu, Japan) according to the manufacturer's protocol. .. Complementary DNA (cDNA) was synthesized from RNA using avian myeloblastosis virus (AMV) reverse transcriptase (BEAMS Biotechnology, Seongnam, South Korea), and random 9-mer primers, then amplified qPCR using primer sets (Takara Bio Inc., Shiga, Japan) specific for mouse COX-2: CAG CAA ATC CTT GCT GTT CC (forward, F) and TGG GCA AAG AAT GCA AAC ATC (reverse, R); mouse iNOS: AGA CCT CAA CAG AGC CCT CA (F) and GGC TGG ACT TTT CAC TCT GC (R); mouse tumor necrosis factor- α (TNF- α ): TCG TAG CAA ACC ACC AAG TG (F) and AGA TAG CAA ATC GGC TGA CG (R); mouse interleukin (IL)-1 β : TCT CGC AGC AGC ACA TCA ACA (F) and CCT GGA AGG TCC ACG GGA AA (R); mouse IL-6: GAC CTG TCT ATA CCA CTT CAC (F) and GTG CAT CAT CGT TGT TCA TAC (R); mouse C-C motif chemokine ligands (CCL) 3: ACT GCC CTT GCT GTT CTT CT (F) and GTC TCT TTG GAG TCA GCG CA (R); mouse CCL4: CTC TCT CCT CTT GCT CGT GG (F) and CTC ACT GGG GTT AGC ACA GA (R); mouse CCL5: CCA TCA TCC TCA CTG CAG CC (F) and CTC TGG GTT GGC ACA CAC TT (R); and mouse C-X-C motif chemokine ligand (CXCL) 10: CCA AGT GCT GCC GTC ATT TT (F) and TCA TCA TTC TTT TTC ATC GTG GCA (R). .. Quantitative real-time PCR was performed using SYBR Green Master Mix (Takara Bio Inc., Tokyo, Japan) on a Takara TP-815 instrument.

    Real-time Polymerase Chain Reaction:

    Article Title: Anti-inflammatory Effects of Empagliflozin and Gemigliptin on LPS-Stimulated Macrophage via the IKK/NF- κ B, MKK7/JNK, and JAK2/STAT1 Signalling Pathways
    Article Snippet: The total RNAs were extracted using the RNAiso Plus reagent (Takara Bio, Otsu, Japan) according to the manufacturer's protocol. .. Complementary DNA (cDNA) was synthesized from RNA using avian myeloblastosis virus (AMV) reverse transcriptase (BEAMS Biotechnology, Seongnam, South Korea), and random 9-mer primers, then amplified qPCR using primer sets (Takara Bio Inc., Shiga, Japan) specific for mouse COX-2: CAG CAA ATC CTT GCT GTT CC (forward, F) and TGG GCA AAG AAT GCA AAC ATC (reverse, R); mouse iNOS: AGA CCT CAA CAG AGC CCT CA (F) and GGC TGG ACT TTT CAC TCT GC (R); mouse tumor necrosis factor- α (TNF- α ): TCG TAG CAA ACC ACC AAG TG (F) and AGA TAG CAA ATC GGC TGA CG (R); mouse interleukin (IL)-1 β : TCT CGC AGC AGC ACA TCA ACA (F) and CCT GGA AGG TCC ACG GGA AA (R); mouse IL-6: GAC CTG TCT ATA CCA CTT CAC (F) and GTG CAT CAT CGT TGT TCA TAC (R); mouse C-C motif chemokine ligands (CCL) 3: ACT GCC CTT GCT GTT CTT CT (F) and GTC TCT TTG GAG TCA GCG CA (R); mouse CCL4: CTC TCT CCT CTT GCT CGT GG (F) and CTC ACT GGG GTT AGC ACA GA (R); mouse CCL5: CCA TCA TCC TCA CTG CAG CC (F) and CTC TGG GTT GGC ACA CAC TT (R); and mouse C-X-C motif chemokine ligand (CXCL) 10: CCA AGT GCT GCC GTC ATT TT (F) and TCA TCA TTC TTT TTC ATC GTG GCA (R). .. Quantitative real-time PCR was performed using SYBR Green Master Mix (Takara Bio Inc., Tokyo, Japan) on a Takara TP-815 instrument.



    Similar Products

    96
    TaKaRa 9 mer primers
    9 Mer Primers, supplied by TaKaRa, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/random+9+mer+primers/Random+Primer/pm41621163-72-13-16
    Average 96 stars, based on 1 article reviews
    9 mer primers - by Bioz Stars, 2026-09
    96/100 stars
      Buy from Supplier

    96
    TaKaRa random 9 mer primers
    Random 9 Mer Primers, supplied by TaKaRa, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/random+9+mer+primers/Random+Primer/pmc12704038-142-9-12
    Average 96 stars, based on 1 article reviews
    random 9 mer primers - by Bioz Stars, 2026-09
    96/100 stars
      Buy from Supplier

    94
    New England Biolabs random 9 mer primers
    Random 9 Mer Primers, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/random+9+mer+primers/Random+Primer+9/pmc12421886-300-15-27
    Average 94 stars, based on 1 article reviews
    random 9 mer primers - by Bioz Stars, 2026-09
    94/100 stars
      Buy from Supplier

    90
    Toyobo random 9-mer primer
    Random 9 Mer Primer, supplied by Toyobo, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/random+9+mer+primers/random+9+mer+primer/pm37666361-71-11-14
    Average 90 stars, based on 1 article reviews
    random 9-mer primer - by Bioz Stars, 2026-09
    90/100 stars
      Buy from Supplier

    94
    New England Biolabs 9 mer primers
    9 Mer Primers, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/random+9+mer+primers/Random+Primer+9/pm37175596-257-16-18
    Average 94 stars, based on 1 article reviews
    9 mer primers - by Bioz Stars, 2026-09
    94/100 stars
      Buy from Supplier

    Image Search Results